[Characterization of the expression of LDH isozymes genes during non-hibernation Himalayan marmots].

Résumé

The lactate dehydrogenase (LDH) isozymes from heart, liver, kidney and skeletal muscle in Himalayan marmots Marmota himalayana robusta) in non-hibernation were investigated in the present experiment. The isozyme patterns were analyzed by means of disc electrophoresis and the ultraviolet enzymatic determination. The following conclusions were obtained: 1. It was found that two expression patterns of LDH genes presented in the skeletal muscle of Himalayan marmots. There was a LDH-B4 band only in one marmots and absent in others. The interindividual polymorphism of LDH isozyme patterns may be attribute to one or more regulator genes controlling synthesis of B-subunit of LDH. 2. LDH zymograms of various tissues of Himalayan marmots showed different subbands. This is presumably due to the existence of both symmetrical and asymmetrical conformers of this tetramer