Article du Bulletin
Expression and purification of woodchuck tumour necrosis factor alpha.
Lohrengel B., Lu M. & Roggendorf M. · 2000 · Cytokine, 12(6): 573-7, ISSN: 1043-4666.
Résumé
The production of recombinant woodchuck cytokines is an essential prerequisite to study the immune response to hepadnavirus infection in the woodchuck model. Woodchuck tumour necrosis factor-alpha (TNF-alpha) was expressed in mammalian cells and in Escherichia coli. A test system for the biological activity of woodchuck TNF-alpha was established on basis of its cytotoxic effect to the murine fibrosarcoma cell line L929. Recombinant TNF-alpha was purified and used for the production of neutralizing antisera.