Variation des propriétés électrocinétiques des noyaux cellulaires de l'épithélium buccal des marmottes en relation avec l'âge et la saison. Season and age-mediated changes in electrokinetic properties of cell nuclei of the marmots buccal epithelium.

Résumé

The method of intracellular microelectrophoresis of cell nuclei was used in investigation of Marmota bobac. The EN% index (per cent of negatively charged nuclei in preparation of native epithelial cells) was used for definition of electrokinetic properties (EKP) of cell nuclei. All the animals were divided into 6 age groups. The EN% values of active marmots in the period from 8 to 18 of June were used for definition of age EKP changes of cellular nuclei. These data show an inverse relation between the EN% index and the age of animals. The data obtained in different season show that there are seasonal changes of EN% index. After the finishing of hibernation the EN% index was max. It remained relatively constant up to autumn, then gradually decreased and had the min value at the beginning of hibernation. Then EN% index increased and reached to the end of hibernation the summer value. It should be noted that the amplitude of seasonal EN% changes of index considerably decreased with the age of animals. During the hibernation the reduction of body mass caused by fat expenditure was accompanied by the increase of EN%.